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anti human cd47  (Elabscience Biotechnology)


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    Elabscience Biotechnology anti human cd47
    Ir1‐PDT downregulates <t>CD47</t> expression and promotes M1 macrophage polarization . (a) CD47 gene expression level (transcripts per million, TPM, n = 3). (b) Representative Western blot images of CD47. Ir1 : 0.1 µM, n = 3. (c) CD47‐SIRPα “Don't Eat Me” Signaling. (d and f) Flow cytometric analysis of CD47 expression. Ir1 : 0.1 µM, n = 3. (e and g) Quantification of CD47 mean fluorescence intensity (n = 3). (h) Experimental flowchart (i‐t). 4T1 cells were cultured (24 h), treated with Ir1 (0.05 µM, 24 h), and irradiated (630 nm, 120 mW cm − 2 , 1 h). RAW264.7 cells were co‐cultured with 4T1 cells and treated for 24 hours prior to sample collection and staining. The flow cytometry dot plot clearly demarcated the differential distribution profiles of RAW264.7 cells (CD45 + ) and 4T1 cells (CD45 − ) in their co‐culture system (n = 3). (i) Flow cytometric analysis of SIRPα expression (n = 3). (j) Quantification of SIRPα mean fluorescence intensity (n = 3). (k) Flow cytometric analysis of phagocytic activity in RAW264.7 cells (n = 3). (l) Statistical analysis of phagocytic activity in RAW264.7 cells (n = 3). (m‐p) Statistical analysis of M1 macrophages (n = 3). (q‐t) Detection of cytokines in cell supernatant (n = 3). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Anti Human Cd47, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human cd47/product/Elabscience Biotechnology
    Average 93 stars, based on 2 article reviews
    anti human cd47 - by Bioz Stars, 2026-05
    93/100 stars

    Images

    1) Product Images from "Ir(III) Complexes Convert Cold to Hot Tumors via Ferroptosis/Necroptosis‐Driven Immunogenic Cell Death and Photosensitized CD47 Downregulation"

    Article Title: Ir(III) Complexes Convert Cold to Hot Tumors via Ferroptosis/Necroptosis‐Driven Immunogenic Cell Death and Photosensitized CD47 Downregulation

    Journal: Advanced Science

    doi: 10.1002/advs.202514256

    Ir1‐PDT downregulates CD47 expression and promotes M1 macrophage polarization . (a) CD47 gene expression level (transcripts per million, TPM, n = 3). (b) Representative Western blot images of CD47. Ir1 : 0.1 µM, n = 3. (c) CD47‐SIRPα “Don't Eat Me” Signaling. (d and f) Flow cytometric analysis of CD47 expression. Ir1 : 0.1 µM, n = 3. (e and g) Quantification of CD47 mean fluorescence intensity (n = 3). (h) Experimental flowchart (i‐t). 4T1 cells were cultured (24 h), treated with Ir1 (0.05 µM, 24 h), and irradiated (630 nm, 120 mW cm − 2 , 1 h). RAW264.7 cells were co‐cultured with 4T1 cells and treated for 24 hours prior to sample collection and staining. The flow cytometry dot plot clearly demarcated the differential distribution profiles of RAW264.7 cells (CD45 + ) and 4T1 cells (CD45 − ) in their co‐culture system (n = 3). (i) Flow cytometric analysis of SIRPα expression (n = 3). (j) Quantification of SIRPα mean fluorescence intensity (n = 3). (k) Flow cytometric analysis of phagocytic activity in RAW264.7 cells (n = 3). (l) Statistical analysis of phagocytic activity in RAW264.7 cells (n = 3). (m‐p) Statistical analysis of M1 macrophages (n = 3). (q‐t) Detection of cytokines in cell supernatant (n = 3). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
    Figure Legend Snippet: Ir1‐PDT downregulates CD47 expression and promotes M1 macrophage polarization . (a) CD47 gene expression level (transcripts per million, TPM, n = 3). (b) Representative Western blot images of CD47. Ir1 : 0.1 µM, n = 3. (c) CD47‐SIRPα “Don't Eat Me” Signaling. (d and f) Flow cytometric analysis of CD47 expression. Ir1 : 0.1 µM, n = 3. (e and g) Quantification of CD47 mean fluorescence intensity (n = 3). (h) Experimental flowchart (i‐t). 4T1 cells were cultured (24 h), treated with Ir1 (0.05 µM, 24 h), and irradiated (630 nm, 120 mW cm − 2 , 1 h). RAW264.7 cells were co‐cultured with 4T1 cells and treated for 24 hours prior to sample collection and staining. The flow cytometry dot plot clearly demarcated the differential distribution profiles of RAW264.7 cells (CD45 + ) and 4T1 cells (CD45 − ) in their co‐culture system (n = 3). (i) Flow cytometric analysis of SIRPα expression (n = 3). (j) Quantification of SIRPα mean fluorescence intensity (n = 3). (k) Flow cytometric analysis of phagocytic activity in RAW264.7 cells (n = 3). (l) Statistical analysis of phagocytic activity in RAW264.7 cells (n = 3). (m‐p) Statistical analysis of M1 macrophages (n = 3). (q‐t) Detection of cytokines in cell supernatant (n = 3). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Techniques Used: Expressing, Gene Expression, Western Blot, Fluorescence, Cell Culture, Irradiation, Staining, Flow Cytometry, Co-Culture Assay, Activity Assay



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    Ir1‐PDT downregulates <t>CD47</t> expression and promotes M1 macrophage polarization . (a) CD47 gene expression level (transcripts per million, TPM, n = 3). (b) Representative Western blot images of CD47. Ir1 : 0.1 µM, n = 3. (c) CD47‐SIRPα “Don't Eat Me” Signaling. (d and f) Flow cytometric analysis of CD47 expression. Ir1 : 0.1 µM, n = 3. (e and g) Quantification of CD47 mean fluorescence intensity (n = 3). (h) Experimental flowchart (i‐t). 4T1 cells were cultured (24 h), treated with Ir1 (0.05 µM, 24 h), and irradiated (630 nm, 120 mW cm − 2 , 1 h). RAW264.7 cells were co‐cultured with 4T1 cells and treated for 24 hours prior to sample collection and staining. The flow cytometry dot plot clearly demarcated the differential distribution profiles of RAW264.7 cells (CD45 + ) and 4T1 cells (CD45 − ) in their co‐culture system (n = 3). (i) Flow cytometric analysis of SIRPα expression (n = 3). (j) Quantification of SIRPα mean fluorescence intensity (n = 3). (k) Flow cytometric analysis of phagocytic activity in RAW264.7 cells (n = 3). (l) Statistical analysis of phagocytic activity in RAW264.7 cells (n = 3). (m‐p) Statistical analysis of M1 macrophages (n = 3). (q‐t) Detection of cytokines in cell supernatant (n = 3). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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    Ir1‐PDT downregulates <t>CD47</t> expression and promotes M1 macrophage polarization . (a) CD47 gene expression level (transcripts per million, TPM, n = 3). (b) Representative Western blot images of CD47. Ir1 : 0.1 µM, n = 3. (c) CD47‐SIRPα “Don't Eat Me” Signaling. (d and f) Flow cytometric analysis of CD47 expression. Ir1 : 0.1 µM, n = 3. (e and g) Quantification of CD47 mean fluorescence intensity (n = 3). (h) Experimental flowchart (i‐t). 4T1 cells were cultured (24 h), treated with Ir1 (0.05 µM, 24 h), and irradiated (630 nm, 120 mW cm − 2 , 1 h). RAW264.7 cells were co‐cultured with 4T1 cells and treated for 24 hours prior to sample collection and staining. The flow cytometry dot plot clearly demarcated the differential distribution profiles of RAW264.7 cells (CD45 + ) and 4T1 cells (CD45 − ) in their co‐culture system (n = 3). (i) Flow cytometric analysis of SIRPα expression (n = 3). (j) Quantification of SIRPα mean fluorescence intensity (n = 3). (k) Flow cytometric analysis of phagocytic activity in RAW264.7 cells (n = 3). (l) Statistical analysis of phagocytic activity in RAW264.7 cells (n = 3). (m‐p) Statistical analysis of M1 macrophages (n = 3). (q‐t) Detection of cytokines in cell supernatant (n = 3). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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    Image Search Results


    Ir1‐PDT downregulates CD47 expression and promotes M1 macrophage polarization . (a) CD47 gene expression level (transcripts per million, TPM, n = 3). (b) Representative Western blot images of CD47. Ir1 : 0.1 µM, n = 3. (c) CD47‐SIRPα “Don't Eat Me” Signaling. (d and f) Flow cytometric analysis of CD47 expression. Ir1 : 0.1 µM, n = 3. (e and g) Quantification of CD47 mean fluorescence intensity (n = 3). (h) Experimental flowchart (i‐t). 4T1 cells were cultured (24 h), treated with Ir1 (0.05 µM, 24 h), and irradiated (630 nm, 120 mW cm − 2 , 1 h). RAW264.7 cells were co‐cultured with 4T1 cells and treated for 24 hours prior to sample collection and staining. The flow cytometry dot plot clearly demarcated the differential distribution profiles of RAW264.7 cells (CD45 + ) and 4T1 cells (CD45 − ) in their co‐culture system (n = 3). (i) Flow cytometric analysis of SIRPα expression (n = 3). (j) Quantification of SIRPα mean fluorescence intensity (n = 3). (k) Flow cytometric analysis of phagocytic activity in RAW264.7 cells (n = 3). (l) Statistical analysis of phagocytic activity in RAW264.7 cells (n = 3). (m‐p) Statistical analysis of M1 macrophages (n = 3). (q‐t) Detection of cytokines in cell supernatant (n = 3). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Advanced Science

    Article Title: Ir(III) Complexes Convert Cold to Hot Tumors via Ferroptosis/Necroptosis‐Driven Immunogenic Cell Death and Photosensitized CD47 Downregulation

    doi: 10.1002/advs.202514256

    Figure Lengend Snippet: Ir1‐PDT downregulates CD47 expression and promotes M1 macrophage polarization . (a) CD47 gene expression level (transcripts per million, TPM, n = 3). (b) Representative Western blot images of CD47. Ir1 : 0.1 µM, n = 3. (c) CD47‐SIRPα “Don't Eat Me” Signaling. (d and f) Flow cytometric analysis of CD47 expression. Ir1 : 0.1 µM, n = 3. (e and g) Quantification of CD47 mean fluorescence intensity (n = 3). (h) Experimental flowchart (i‐t). 4T1 cells were cultured (24 h), treated with Ir1 (0.05 µM, 24 h), and irradiated (630 nm, 120 mW cm − 2 , 1 h). RAW264.7 cells were co‐cultured with 4T1 cells and treated for 24 hours prior to sample collection and staining. The flow cytometry dot plot clearly demarcated the differential distribution profiles of RAW264.7 cells (CD45 + ) and 4T1 cells (CD45 − ) in their co‐culture system (n = 3). (i) Flow cytometric analysis of SIRPα expression (n = 3). (j) Quantification of SIRPα mean fluorescence intensity (n = 3). (k) Flow cytometric analysis of phagocytic activity in RAW264.7 cells (n = 3). (l) Statistical analysis of phagocytic activity in RAW264.7 cells (n = 3). (m‐p) Statistical analysis of M1 macrophages (n = 3). (q‐t) Detection of cytokines in cell supernatant (n = 3). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: From BioLegend (San Diego, CA), we acquired TruStain FcXTM (anti‐mouse CD16/32, 101 319), APC anti‐mouse CD47 (127 514), APC anti‐mouse CD80 (104 714), PE anti‐mouse CD86 (105 008), FITC anti‐mouse CD3 (100 204), BV421 anti‐mouse CD4 (562 891), PE anti‐mouse CD4 (100 408), FITC anti‐mouse CD206 (141 703), APC anti‐mouse CD8a (162 306), Alexa Fluor® 700 anti‐mouse CD45 (103 128), and FITC anti‐mouse CD11c (117 305), while FITC anti‐SIRPα/SHPS‐1 was purchased from Sino Biological (Beijing, China) and PE anti‐human CD47 (E‐AB‐F1413D) from Elabscience (Wuhan, China).

    Techniques: Expressing, Gene Expression, Western Blot, Fluorescence, Cell Culture, Irradiation, Staining, Flow Cytometry, Co-Culture Assay, Activity Assay

    Immunophenotyping panel for multiplexed tissue imaging of cancer.

    Journal: Frontiers in Immunology

    Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

    doi: 10.3389/fimmu.2024.1383932

    Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

    Article Snippet: CD47 , REA220 , 50 , 130-123-754 , PE , Miltenyi Biotec.

    Techniques: Imaging